The Bulls travel to the Memorial Ground for the first time since Rovers moved into the stadium in 1996 after an eleven year stay at Bath's Twerton Park.
Despite the rivalry, it's 19 years since the two sides last met in a League Cup two-legged encounter with Rovers winning the match at Twerton Park 1-0, but the Bulls won the return leg 2-0 to edge past the higher graded side.
It's nearly 30 years since a league encounter, with Rovers failing to beat the Bulls in four attempts in the league. A 1-1 draw at Edgar Street in April 1977 in what is now League One being the last league meeting.
The Bulls will look to improve on a recent run of just one win in the last six games. A more controlled performance against Wrexham failed to have an end product attached to it and a defeat to Rovers will see the Bulls slip into the bottom half of the table.
Rovers have been described by their manager as unlucky or just plain awful in recent weeks as they have gone five matches without a win. Despite spending £200,000 on Rochdale striker Rickie Lambert they have struggled to find the net with consistency, failing to find the net more than once in any game this season.
Bristol Rovers vs Hereford United, Memorial Ground, Wednesday September 27th, 7.45pm.